Thermo Fisher Scientific Alpha A Crystallin Monoclonal Antibody (1H3.B8), APC
Alpha A Crystallin 단백질을 인식하는 마우스 단클론 항체로, APC 형광단백질로 결합됨. Western blot, ICC/IF, ELISA에 사용 가능. 높은 특이성과 재현성을 제공하며, 다양한 종에 반응함. 연구용으로 렌즈 단백질 및 스트레스 반응 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H3.B8 |
| Immunogen | Native Alpha Crystallin |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931626 |
Additional Formats
- Unconjugated (MA5-27582)
- FITC (MA5-45173)
- PE (MA5-45175)
- PerCP (MA5-45174)
- Custom conjugation available upon request
Product Specific Information
0.5 µg/mL was sufficient for detection of 100 ng purified alphaA crystallin by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Detects approximately 20 kDa.
Does not cross-react with alpha B-crystallin, beta L-crystallin, beta H-crystallin, gamma-crystallin, HSP25, HSP27, or HSP47 proteins.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%), which are also found in vertebrate skeletal muscle tissue.
In the lens, crystallins maintain the proper refractive index. The mammalian lens contains three major classes: alpha, beta, and gamma.
Alpha-crystallin is the largest, composed of alpha-A and alpha-B subunits, and makes up about 40% of total lens protein.
It acts as a molecular chaperone, preventing aggregation that could lead to cataract formation.
Phosphorylated alpha-crystallin may serve a structural control function and play a role in protein maintenance.
Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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