Thermo Fisher Scientific POLR2A Polyclonal Antibody
POLR2A 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, ICC/IF, ELISA, IP 등에 사용 가능. 인간, 마우스, 랫트 반응성. 액상 형태로 1.87 mg/mL 농도, -20°C 보관. 핵 내 POLR2A 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–260 of human POLR2A (NP_000928.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.87 mg/mL |
| Purification | Affinity Chromatography |
| Storage buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2914360 |
Product Specific Information
- Positive test controls: HeLa, Jurkat, Mouse brain
- Subcellular localization: Nucleus
Immunogen sequence:
MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIEL AKPVFHVGFL VKTMKVLRCV CFFCSKLLVD SNNPKIKDIL AKSKGQPKKR LTHVYDLCKG KNICEGGEEM DNKFGVEQPE GDEDLTKEKG HGGCGRYQPR IRRSGLELYA EWKHVNEDSQ EKKILLSPER VHEIFKRISD EECFVLGMEP RYARPEWMIV TVLPVPPLSV
Target Information
DNA-directed RNA polymerase II subunit RPB1 (POLR1A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates. POLR1A is the largest subunit and a catalytic component of RNA polymerase II, which synthesizes mRNA precursors and non-coding RNAs. It forms the polymerase active center with the second largest subunit.
Pol II is the central component of the basal RNA polymerase II transcription machinery, composed of mobile elements that move relative to each other. RPB1 contributes to the core element with the central cleft, clamp, and jaws that interact with the DNA template.
During transcription initiation, the promoter DNA strand is positioned within the active site cleft. A bridging helix from RPB1 promotes translocation of Pol II by switching conformations, facilitating nucleotide addition. The phosphorylation status of the C-terminal domain (CTD) regulates transcription initiation, elongation, termination, and mRNA processing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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