Thermo Fisher Scientific TRIM25 Polyclonal Antibody
TRIM25 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot 및 IHC(P)에서 사용 가능하며, Human과 Rat에 반응. 항원 친화 크로마토그래피로 정제된 액상 형태로, 세포 내 TRIM25 단백질 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within human TRIM25 aa 20–320 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2913376 |
Product Specific Information
- Positive Control: Jurkat cell lysate, 293 cell lysate, SK-Br-3 cell lysates, rat bladder tissue
- Subcellular Location: Cytoplasm, Stress granule
Target Information
TRIM25, also known as estrogen responsive finger protein (EFP), is a member of the RING finger-B box-coiled coil family and a downstream target of estrogen receptor α.
Mice lacking this gene display an underdeveloped uterus and reduced estrogen responsiveness, indicating that TRIM25 is essential for estrogen-dependent proliferation.
TRIM25 expression is increased in breast carcinoma, promoting tumor growth by functioning as an E3 ubiquitin ligase targeting the negative cell cycle checkpoint 14-3-3σ.
Additionally, TRIM25 E3 ubiquitin ligase is crucial for the retinoic acid-inducible gene 1 (RIG-1) signaling pathway, which mediates host antiviral innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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