Thermo Fisher Scientific HUNK Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic 19 amino acid peptide from C-terminal cytoplasmic domain of human HUNK. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HUNK,uniProtId:P57058-1,ncbiNodeId:9606,antigenRange:714,antigenLength:714,antigenImageFileName:PA5-32872_HUNK_P57058-1_Rabbit.svg,antigenImageFileNamePDP:PA5-32872_HUNK_P57058-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2550333

Product Specific Information

Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Bovine, Elephant (100%); Rabbit (95%); Gibbon, Marmoset, Dog, Panda, Horse (89%); Mouse, Rat, Hamster (84%).

For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

HUNK serine/threonine kinase or hormonally up-regulated Neu-associated kinase is a member of the mitogen-activated protein kinase family which is essential for mammary tumor metastasis. HUNK deletion in mice did not alter viability, fertility, longevity, or propensity to develop tumors and it is also dispensable for murine development. HUNK has been shown to participate in vesicular transport. Additionally, functional studies in mice suggest that HUNK regulates endocytosis via interaction with rabaptin-5 protein and induces changes in mammary glands during pregnancy. HUNK expression has been documented in mouse brain, breast, embryo, and fetus. ESTs have been isolated from several human tissue libraries, including normal ear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.