Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Alexa Fluor 532, eBioscience
Mouse CD45 단백질을 인식하는 30-F11 클론의 Rat IgG2b 모노클론 항체로, Alexa Fluor 532 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 모든 혈액세포에서 CD45 검출에 사용됩니다. 높은 특이성과 안정적인 형광 신호 제공.
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Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Alexa Fluor 532, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 40 publications |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunohistochemistry (Frozen) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 10 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 218 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 532 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F11 |
| Conjugate | Alexa Fluor 532 |
| Excitation / Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219868 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV, Violet, Yellow series
- FITC, PE, PE-Cyanine, PerCP-Cyanine
- Super Bright, NovaFluor series
- Functional Grade
- Custom conjugation available
Product Specific Information
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. Its cytoplasmic domain exhibits tyrosine phosphatase activity and plays a key role in lymphocyte activation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.
Fluorophore Information:
Alexa Fluor® 532 is excited by a Green laser (532 nm) and emits at 561 nm. Not compatible with a Yellow-Green laser (561 nm). Recommended filter: 560/14 band pass.
Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser.
Target Information
CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). It is absent in non-hematopoietic tissues. CD45 regulates Src-family kinases, T/B-cell receptor signaling, cytokine receptor signaling, and integrin-mediated pathways. Multiple isoforms arise from alternative splicing, differing in extracellular domains. CD45 is essential for lymphocyte development and immune function regulation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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