Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Alexa Fluor 532, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Alexa Fluor 532, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.125 µg)/test
  View 4 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 561, 647, 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV/Violet/Yellow series
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine series
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series
• NovaFluor Blue/Red/Yellow variants
• Functional Grade
• Request custom conjugation

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha).
CD127 complexes with CD132 (common gamma chain, γc) to form the IL-7 receptor (IL-7R), a type I glycoprotein (75–80 kDa). It is expressed on immature B cells (early pre-B stage), thymocytes, and most mature T cells, with transient down-regulation upon activation.

IL-7 binding triggers signal transduction via tyrosine kinase pathways, including Jak/STAT, essential for lymphocyte development and homeostatic T-cell proliferation.
IL-7R signaling also regulates TCR locus rearrangement in γδ T cells.

CD127 expression is notably down-regulated on CD4+CD25+ regulatory T cells (Tregs). Cells co-expressing CD4 and CD25 but lacking CD127 are highly enriched for Tregs, defined by Foxp3 expression and in vitro suppressive activity.

Binding of eBioRDR5 to PBMCs is blocked by pre-incubation with recombinant human IL-7 (Product #14-1079-80).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on human peripheral blood cells
Use at 5 µL (0.125 µg)/test (100 µL final volume, 10⁵–10⁸ cells/test)

Fluorochrome Properties:

• Excitation: 532 nm
• Emission: 561 nm
• Laser: Green laser (not compatible with Yellow-Green laser)
• Recommended filter: 560/14 band pass

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein regulating lymphopoiesis.
Its receptor (IL7R) is an alpha/gamma chain heterodimer.

• Gamma(c) chain: Activates signal transduction
• Alpha chain: Determines specific signaling events

CD127 promotes proliferation of precursor B cells, thymocytes, T-cell progenitors, and mature CD4+/CD8+ T cells.
Dysfunction is linked to severe combined immunodeficiency (SCID) and T-cell negative/B-cell negative/NK positive SCID.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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