Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Alexa Fluor 532, eBioscience
인간 CD45 모든 아이소폼을 인식하는 HI30 단클론 항체로, Alexa Fluor 532 형광 표지. Flow cytometry에 최적화되어 있으며, 532 nm excitation/561 nm emission 특성. 면역세포 활성화 연구 및 림프구 분석용.
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Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Alexa Fluor 532, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | - | View 7 publications |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 45 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 532 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI30 |
| Conjugate | Alexa Fluor™ 532 |
| Excitation / Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218084 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV / Violet / NovaFluor / PE / PerCP series 다양하게 제공
- Request custom conjugation
Product Specific Information
The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except erythrocytes and platelets. Its cytoplasmic domain has tyrosine phosphatase activity, playing a key role in lymphocyte activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for flow cytometry on normal human peripheral blood cells
- Recommended Usage: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Fluorophore Information:
Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. Not compatible with Yellow-Green laser (561 nm). Recommended filter: 560/14 band pass.
Optical Properties:
Excitation: 532 nm
Emission: 561 nm
Laser: Green laser
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, representing ~10% of lymphocyte surface proteins. It is absent in non-hematopoietic tissues and cell lines. CD45 plays essential roles in lymphocyte development, antigen signaling, and regulation of Src-family kinases. Isoforms arise via alternative splicing, differing in extracellular domains. CD45RA and other isoforms show cell-type-specific expression and functional diversity. It regulates T/B-cell receptor signaling, cytokine receptor pathways, and integrin-mediated survival signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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