Thermo Fisher Scientific Phospho-Zap-70 (Tyr319, Tyr352) Monoclonal Antibody (GT1686)
ZAP-70 인산화 형태(Tyr319, Tyr352)를 특이적으로 인식하는 단클론 항체. Western blot 및 면역세포염색에 적합. 인간 시료 반응성. 단백질 G 정제, 1 mg/mL 농도, 보존제 무첨가. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT1686 |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human ZAP70 (phospho Tyr319)/Syk (phospho Tyr352). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2866543 |
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase and a member of the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319. Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70 function in lymphocyte signaling, while phosphorylation at tyrosine 292 attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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