Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), eFluor 660, eBioscience
Rat IgG1 kappa 단클론 동종 대조항체(eBRG1)로, eFluor 660 형광체가 결합된 제품입니다. Flow cytometry 및 IHC 실험에서 음성 대조용으로 사용됩니다. 651/668 nm 파장 특성을 가지며, 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBRG1 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597289 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
Applications Reported:
eFluor® 660 Rat IgG1 Isotype Control has been reported for use in flow cytometric analysis and immunohistochemical staining.
Applications Tested:
Tested by flow cytometric analysis of mouse spleen cells and normal human peripheral blood lymphocytes. The isotype control should be used at the same concentration as the experimental antibody.
Fluorochrome Information:
eFluor® 660 is a replacement for Alexa Fluor® 647. It emits at 659 nm and is excited with the red laser (633 nm). Ensure that your instrument is capable of detecting this fluorochrome.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies commonly used in research and diagnostics. These antibodies, produced by rats in response to an antigen, can be purified from serum or hybridoma culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is widely applied in immunohistochemistry, flow cytometry, and western blotting for antigen detection.
Gene targeting in rats enables the study of human disease mechanisms by allowing specific gene knockout, insertion, or modification.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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