Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660, eBioscience
eFluor 660으로 표지된 Rat IgG2a kappa 동형 대조 항체로, 면역세포염색(IHC, ICC/IF) 및 유세포분석(Flow Cytometry)에 사용됩니다. Alexa Fluor 647 대체 형광체 사용, 651/668 nm 파장 특성. 4°C 보관, 연구용 전용 시약.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBR2a |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10598640 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor 450, 506, 570, 615
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780 series
- Custom conjugation available on request
Product Specific Information
Description:
This monoclonal Rat IgG2a antibody serves as an isotype control immunoglobulin.
Applications Reported:
eFluor® 660 Rat IgG2a Isotype Control is suitable for immunocytochemistry, immunohistochemistry, and flow cytometry.
Applications Tested:
Validated for flow cytometric analysis of mouse splenocytes. Use at the same concentration as the experimental antibody.
Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647. It emits at 659 nm and is excited with a red laser (633 nm). Ensure instrument compatibility.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Rat IgG2a is an immunoglobulin subclass used in research and diagnostics. It activates complement and mediates ADCC.
Molecular weight ≈ 160 kDa, composed of two heavy and two light chains.
Commonly used as a control or reference antibody in rat model experiments, and in flow cytometry and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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