Thermo Fisher Scientific EPN2 Monoclonal Antibody (OTI1H3), TrueMAB
EPN2 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF에 적합합니다. 인간 시료 반응성이 있으며, 동결건조 형태로 제공됩니다. 클라트린 매개 내포작용 연구에 활용 가능하며, 재구성 및 결합 실험에 최적화되어 있습니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H3 |
| Immunogen | Full length human recombinant protein of human EPN2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Epsin2 is involved in the formation of clathrin-coated invaginations and endocytosis. It interacts with EPS15 via the NPF repeat domain and with AP-2 and clathrin via the DPW repeat domain.
The protein is localized in cytoplasmic punctate structures associated with clathrin-coated vesicles and concentrated near the Golgi complex.
Highest expression is observed in the brain, with lower levels in lung and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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