Thermo Fisher Scientific FMR1 Monoclonal Antibody (OTI1C6), TrueMAB
FMR1 단백질을 인식하는 고특이성 단클론 항체로 Western blot, IHC, ICC, Flow cytometry에 사용 가능. 인간, 마우스, 랫, 개 시료 반응성. 동결건조 형태로 안정적이며 연구용 mRNA 관련 단백질 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 36–279 of human FMR1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting tool: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
FMR1 binds RNA and associates with polysomes, potentially involved in mRNA trafficking from the nucleus to the cytoplasm.
A trinucleotide repeat in the 5′ UTR normally ranges from 6–53 copies; expansion to 55–230 repeats causes fragile X syndrome.
Repeat expansion may also lead to premature ovarian failure.
Multiple alternatively spliced transcript variants produce different isoforms with distinct cellular localizations.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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