Thermo Fisher Scientific BTLA Monoclonal Antibody (OTI2H5), TrueMAB
BTLA 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot 및 유세포분석에 적합합니다. 동결건조 형태로 제공되며, 재구성 시 약 1 mg/mL 농도로 사용 가능합니다. 면역억제 수용체 연구 및 림프구 활성 조절 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 31–157 + Mouse Fc of human BTLA produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
BTLA (B and T-lymphocyte attenuator) is a co-inhibitory receptor belonging to the CD28 superfamily.
It functions as a lymphocyte inhibitory receptor that downregulates immune responses.
BTLA is constitutively expressed on most CD4+ and CD8+ T cells, with expression decreasing upon T cell activation.
It remains expressed on Th1 cells but not Th2 cells, and interacts with HVEM (herpesvirus entry mediator), a TNF receptor superfamily member.
This interaction plays an important role in regulating lymphocyte activation and immune homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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