Thermo Fisher Scientific VEGF Monoclonal Antibody (OTI4G3), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution

• 1:2500–1:5000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

VEGF (vascular endothelial growth factor) is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis, promoting tumor progression and metastasis.
VEGF affects vascular endothelium by enhancing cell viability, mitogenesis, chemotaxis, and vascular permeability.
The VEGF family includes VEGF-A, -B, -C, -D, -E, and PIGF. VEGF and its receptor system are key regulators of angiogenic signaling.
VEGF isoforms, generated by alternative splicing, bind to tyrosine kinase receptors FLT1 (VEGFR-1) and FLK1/KDR (VEGFR-2), primarily expressed on endothelial cells.
Soluble FLT1 (sFLT1) binds VEGF with high affinity, inhibiting angiogenesis.
Elevated VEGF levels are associated with POEMS syndrome (Crow-Fukase syndrome), affecting multiple organs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.