Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7)
DYKDDDDK(FALG) 태그 단백질 검출용 마우스 단클론 항체. WB, IHC, ELISA, IP 등 다양한 실험에 적용 가능. FLAG 태그가 부착된 재조합 단백질의 특이적 검출에 적합. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:150,000–1:250,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29E4.G7 |
| Immunogen | Synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Agarose
- Biotin
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 649
- DyLight 680
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze–thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
The purified antibody is directed against the FLAG™ motif and is useful in detecting its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal anti-FLAG™ tag antibody detects overexpressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Target Information
The DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag to facilitate purification, detection, and localization. It consists of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) and can be fused to the N- or C-terminus of a protein of interest. The FLAG tag allows easy identification and isolation of tagged proteins using anti-FLAG antibodies. It is widely used in molecular biology and biochemistry for protein-protein interaction studies, expression analysis, and localization studies. The FLAG tag is hydrophilic, minimizing interference with protein folding or activity.
FLAG™ is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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