Thermo Fisher Scientific Phospho-Stat5 (Tyr694) Monoclonal Antibody (5F6.F1)
STAT5 단백질의 Tyr694 인산화를 특이적으로 인식하는 단클론 항체. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-Stat5 (Tyr694) Monoclonal Antibody (5F6.F1)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 20–40 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:50–1:1,000 |
| ELISA | 1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F6.F1 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- For long-term storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody is specific for mouse Stat5a protein phosphorylated at Y694.
BLAST analysis indicates cross-reactivity with Stat5a from human, mouse, and rat based on 100% homology with the immunizing sequence.
Cross-reactivity with other species has not been determined.
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT proteins are phosphorylated by receptor-associated kinases and form homo- or heterodimers that translocate to the nucleus to act as transcription activators.
STAT5 alpha mediates responses to ligands such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and various growth hormones.
Activation of STAT5 alpha in myeloma and lymphoma associated with TEL/JAK2 gene fusion is independent of cell stimuli and is essential for tumorigenesis.
In mice, STAT5 alpha induces BCL2L1/BCL-X(L) expression, suggesting an antiapoptotic role.
STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues, with differential expression in muscle and mammary tissue.
STAT5 alpha plays critical roles in reproduction, lactation, immune function, and growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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