Thermo Fisher Scientific ETS1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human ETS1 protein. Validated for WB, ICC/IF, and IP applications. Recognizes cytoplasmic and nuclear ETS1. Supplied as liquid, unconjugated form, 0.933 mg/mL concentration. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–260 of human ETS1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.933 mg/mL |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805387 |
Product Specific Information
Immunogen sequence:
MKAAVDLKPT LTIIKTEKVD LELFPSPDME CADVPLLTPS SKEMMSQALK ATFSGFTKEQ QRLGIPKDPR QWTETHVRDW VMWAVNEFSL KGVDFQKFCM NGAALCALGK DCFLELAPDF VGDILWEHLE ILQKEDVKPY QVNGVNPAYP ESRYTSDYFI SYGIEHAQCV PPSEFSEPSF ITESYQTLHP ISSEELLSLK YENDYPSVIL RDPLQTDTLQ NDYFAIKQEV VTPDNMCMGR TSRGKLGGQD SFESIESYDS
Positive Samples: U-937, Raji, Jurkat, ES-2
Cellular Location: Cytoplasm, Nucleus
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid protein and a member of the DNA-binding ETS protein family. It is a multifunctional protein containing an N-terminal pointed domain, a central transactivation domain, and two auto-inhibitory domains flanking a conserved C-terminal ETS domain.
ETS1 interacts with factors such as PEBP2alphaA and p300/CBP, mediating transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase. It is activated by Ras/MAPK signaling and is upregulated in resting T-cells. Its activity is modulated by AP-1, Sp-1, c-myb, and MafB. Sp100 activates ETS1 in nuclear bodies. ETS1 interacts with EAP1/Daxx to repress MMP1 and BCL2 transactivation, and with STAT6 to modulate Socs-1 expression induced by IL-4. It also interacts with GATA3 to positively regulate Tax-1 dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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