Thermo Fisher Scientific Eotaxin 2 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific Eotaxin 2 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine Eotaxin-2 (CCL24). Anti-Murine Eotaxin-2 (CCL24)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mEotaxin-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Eotaxin-2 (500-P175G) as a capture antibody, enables detection of at least 0.2–0.4 ng/well of recombinant mEotaxin-2.

Western Blot:
For detection of mEotaxin-2 by Western Blot, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for recombinant mEotaxin-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P175GBT-1MG will be provided as 2 × 500 µg.

Target Information

Eotaxin 2 belongs to the subfamily of small cytokine CC genes. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. CC cytokines are characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, minimal activity on neutrophils, and no activity on monocytes and activated T lymphocytes. It is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.