Thermo Fisher Scientific HLA-DRB (MHC II) Monoclonal Antibody (HLA-DRB, 1067)
HLA-DRB(MHC II) 단일클론 항체로 인간 및 비인간 영장류 반응성. WB, IHC, ICC, Flow 등 다양한 응용 가능. Protein A/G 정제, 액상 형태, 4°C 보관. 항원제시세포 표면의 HLA-DR 베타 사슬 인식용 연구용 항체.
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Thermo Fisher Scientific HLA-DRB (MHC II) Monoclonal Antibody (HLA-DRB, 1067)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–4 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HLA-DRB, 1067 |
| Immunogen | Activated human peripheral blood mononuclear cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 min.
Target Information
HLA-DR beta refers to the beta chain of the HLA-DR protein complex, a member of the human leukocyte antigen (HLA) class II molecule. The HLA-DR beta chain is encoded by the HLA-DRB gene and is primarily expressed on the surface of antigen-presenting cells, including dendritic cells, macrophages, and B cells.
These cells play a crucial role in the immune system by capturing, processing, and presenting antigens to T cells for immune recognition. HLA-DR beta facilitates recognition and response to extracellular antigens, presenting antigenic peptides to CD4+ T cells and activating adaptive immune responses.
Structurally, HLA-DR beta consists of:
- Extracellular domain: contains the peptide-binding groove interacting with antigenic peptides
- Transmembrane domain: anchors the molecule to the cell membrane
- Cytoplasmic tail: involved in intracellular signaling
HLA and MHC antibodies are essential tools in Immunopeptidomics, enabling identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 3123-MSM2-P1_HLA-DR_beta_P01911-1_House_mouse.svg, 3123-MSM2-P1_HLA-DR_beta_P01911-1_House_mouse_PDP.jpeg, 3123-MSM2-P1_HLA-DR_beta_P13762-1_House_mouse.svg, 3123-MSM2-P1_HLA-DR_beta_P13762-1_House_mouse_PDP.jpeg, 3123-MSM2-P1_HLA-DR_beta_P79483-1_House_mouse.svg, 3123-MSM2-P1_HLA-DR_beta_P79483-1_House_mouse_PDP.jpeg, 3123-MSM2-P1_HLA-DR_beta_Q30154-1_House_mouse.svg, 3123-MSM2-P1_HLA-DR_beta_Q30154-1_House_mouse_PDP.jpeg)
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