Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:400

-

Immunocytochemistry (ICC/IF)

1:800

View 9 publications 9 publications

Flow Cytometry (Flow)

1:50

View 1 publication 1 publication

Peptide Array (Array)

1:2,000

-

Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Non-human primate, Rat, Xenopus

Published species

C. elegans, Chicken, Fruit fly, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer10 of human histone H3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Histone H3,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:10,antigenLength:136,antigenImageFileName:PA5-17869_Histone_H3_P68431-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17869_Histone_H3_P68431-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Histone H3,uniProtId:Q71DI3-1,ncbiNodeId:9606,antigenRange:10,antigenLength:136,antigenImageFileName:PA5-17869_Histone_H3_Q71DI3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-17869_Histone_H3_Q71DI3-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 100µg/mL BSA, 0.15M NaCl, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984484

Product Specific Information

Phospho-Histone H3 (Ser10) Antibody detects endogenous levels of histone H3 only when phosphorylated at Ser10; however, this antibody does not detect phosphorylated Ser10 when Lys9 is acetylated or methylated. This antibody does not cross-react with histone H3 phosphorylated at Ser28.

This antibody is not cross-reactive with other phosphorylated histones or with acetylated histones.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.