Thermo Fisher Scientific Reactive Oxygen Species (ROS) Fluorometric Assay Kit
세포 및 조직 내 활성산소종(ROS)을 정량 측정하는 형광 기반 어세이 키트. DCFH-DA 프로브 사용으로 높은 민감도와 재현성 제공. 간단하고 빠른 프로토콜로 30~60분 내 결과 확인 가능. 다양한 장비와 샘플 유형에 호환.
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Invitrogen™ Reactive Oxygen Species (ROS) Fluorometric Assay Kit
The Reactive Oxygen Species (ROS) Fluorometric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ROS in fresh tissue and live cell samples.
ROS are highly reactive molecules that play a crucial role in various physiological and pathological processes, including oxidative stress, inflammation, and cellular signaling.
Assay Principle
The assay utilizes the fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) to detect ROS.
DCFH-DA is taken up by cells and converted to dichlorofluorescein (DCFH) by intracellular esterase. DCFH is oxidized by ROS to the strongly fluorescent DCF.
The fluorescence of DCF (Ex/Em 502/525 nm) is directly proportional to the amount of ROS present in the sample.
Key Features
- Simple and fast protocol requiring minimal hands-on time
- Provides rapid results within a short incubation period
- Broad range of kits for key analytes and metabolic targets
- Consistent and reproducible results through strict quality control
Specifications
| 항목 | 내용 |
|---|---|
| 검출 방법 | Fluorescence |
| 샘플 종류 | Cell Culture, Live Tissue |
| 사용 장비 | Spectrophotometer, Microplate Reader, Flow Cytometer |
| 인큐베이션 시간 | 30–60 min |
| 총 어세이 시간 | 60 min |
| 수량 | 96 tests |
| 배송 조건 | Dry Ice, 2–8°C |
| 타겟 | Reactive Oxygen Species (ROS) |
| Unit Size | Each |
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