Invitrogen™

Lactate Dehydrogenase (LDH) Colorimetric Activity Kit

The Lactate Dehydrogenase (LDH) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of자세히 알아보기

The Lactate Dehydrogenase (LDH) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of LDH in serum, plasma, tissue, and cell culture samples. LDH is an enzyme that catalyzes the conversion of lactate to pyruvate, an essential step in cellular energy metabolism. Elevated LDH levels are associated with tissue damage, inflammation, and certain diseases, making it a useful biomarker in clinical and research settings.

In this assay, LDH catalyzes lactic acid to produce pyruvate. Pyruvate reacts with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which is red-brown in alkaline solution. The color intensity is proportional to pyruvate concentration, where the activity of LDH can be calculated by measuring OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

사양

어세이 범위6–1000 U/L

어세이 민감성6 U/L

검출 방법Colorimetric

샘플 종류Hydrothorax, Plasma, Serum, Tissue Homogenates, Whole Cells

용도(장비)Microplate Reader

인큐베이션 시간35 min.

분석 간 CV2.4

분석 내 CV1.8

수량96 tests

샘플 부피40 μL

배송 조건Dry Ice, 2–8°C

타겟 키트 이름 지정Lactate Dehydrogenase (LDH)

총 어세이 시간45 min.

Unit SizeEach