Invitrogen™

Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

The Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative자세히 알아보기

The Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of NEFA in animal tissue samples. NEFAs are derived from adiopose tissue, where hormone-sensitive lipase decomposes triglycerides to product fatty acids. These acids circulate freely in the body and, when combined with plasma albumin, can be used as an energy sourced for muscles, organs, and the brain. They are also a substrate of fat synthesis, where the concentration of NEFAs is related to metabolism and endocrine function.

In this assay NEFA react with nantokite to form copper soap, which has a specific absorption peak at 715 nm. The content of NEFA can be calculated indirectly by measuring the OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

사양

어세이 범위0.15–1.5 mmol/L

어세이 민감성0.15 mmol/L

검출 방법Colorimetric

샘플 종류Tissue Homogenates

용도(장비)Microplate Reader

인큐베이션 시간3 min.

분석 간 CV5.1

분석 내 CV3.3

수량96 tests

샘플 부피1000 μL

배송 조건Dry Ice, 2–8°C

타겟 키트 이름 지정Non-esterified Free Fatty Acids (NEFA/FFA)

총 어세이 시간30 min.

Unit SizeEach