Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), FITC, eBioscience
마우스 CD106(VCAM-1)을 인식하는 단클론 항체로, FITC 형광 표지되어 플로우 사이토메트리 분석에 적합합니다. 0.5 µg/test 농도로 사용 가능하며, 염증 반응 및 세포 부착 연구에 활용됩니다. 4°C 암조건 보관, 동결 금지.
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Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (Clone 429), FITC, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 17 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 429 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465180 |
Available Formats
- Unconjugated
- APC
- Biotin
- eFluor 450
- eFluor 660
- Functional Grade
- PE
- PE-Cyanine7
- Request custom conjugation available
Product Specific Information
The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation.
CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), which are important for early lymphocyte and myeloid development in the bone marrow and thymus.
Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role in inflammatory response.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤ 0.5 µg per test (100 µL final volume). Optimal titration recommended.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, adhesion, and signal transduction, and may play a role in inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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