Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), FITC, eBioscience

Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The eBioH4A3 monoclonal antibody reacts with human CD107a (LAMP-1), a highly glycosylated protein (~110 kDa) predominantly expressed in lysosomal/endosomal membranes. CD107a transiently appears on the surface of degranulating cytolytic T cells and is upregulated on activated platelets and certain cancer cells.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis
• Surface staining in flow cytometric degranulation assays

Applications Tested:
Pre-titrated and validated for intracellular staining and flow cytometric analysis of Jurkat cells.
Use at 5 µL (0.5 µg) per test (final volume 100 µL). Cell number per test: 10⁵–10⁸ cells.

Fluorescence Properties:

• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

LAMP1 (CD107a) is a major lysosomal membrane component alongside LAMP2. About 1–2% of CD107a is found on the plasma membrane. It is a type I integral membrane protein with a signal peptide, 18 N-linked glycosylation sites, a single transmembrane segment, and a short cytosolic tail.

Biological Role:

• Involved in lysosome biogenesis and fusion with phagosomes
• Transports from trans-Golgi network to endosomes and lysosomes
• Surface expression upon cell activation depends on a carboxyl-terminal tyrosine motif (YXXI)
• Mutations in this motif affect lysosomal localization and membrane cycling
• Promotes PBMC adhesion to vascular endothelium, suggesting a role in inflammation
• Increased expression in neurons and glial cells near senile plaques in Alzheimer’s disease
• Marker of mast cell activation

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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