Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), FITC, eBioscience
CD107a (LAMP-1) 단일클론 항체 eBioH4A3, FITC 형광표지로 인간 세포의 CD107a 검출에 최적화됨. Flow cytometry 및 intracellular staining에 검증됨. 488 nm/520 nm 스펙트럼, 고순도 친화 크로마토그래피 정제. 연구용으로만 사용.
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- 11-1079-4x (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 0.5 µg/mL | View 8 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 23 publications |
| Inhibition Assays (IA) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH4A3 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11040009 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The eBioH4A3 monoclonal antibody reacts with human CD107a (LAMP-1), a highly glycosylated protein (~110 kDa) predominantly expressed in lysosomal/endosomal membranes. CD107a transiently appears on the surface of degranulating cytolytic T cells and is upregulated on activated platelets and certain cancer cells.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
- Surface staining in flow cytometric degranulation assays
Applications Tested:
Pre-titrated and validated for intracellular staining and flow cytometric analysis of Jurkat cells.
Use at 5 µL (0.5 µg) per test (final volume 100 µL). Cell number per test: 10⁵–10⁸ cells.
Fluorescence Properties:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
LAMP1 (CD107a) is a major lysosomal membrane component alongside LAMP2. About 1–2% of CD107a is found on the plasma membrane. It is a type I integral membrane protein with a signal peptide, 18 N-linked glycosylation sites, a single transmembrane segment, and a short cytosolic tail.
Biological Role:
- Involved in lysosome biogenesis and fusion with phagosomes
- Transports from trans-Golgi network to endosomes and lysosomes
- Surface expression upon cell activation depends on a carboxyl-terminal tyrosine motif (YXXI)
- Mutations in this motif affect lysosomal localization and membrane cycling
- Promotes PBMC adhesion to vascular endothelium, suggesting a role in inflammation
- Increased expression in neurons and glial cells near senile plaques in Alzheimer’s disease
- Marker of mast cell activation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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