Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), FITC, eBioscience

Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• eFluor 450
• eFluor 660
• PE
• Custom conjugation available

Product Specific Information

The eBioH4B4 monoclonal antibody reacts with human CD107b (LAMP-2), a highly glycosylated type I transmembrane protein (~105 kDa) expressed in lysosomal/endosomal membranes. It is found on degranulating T cells, activated platelets, and some cancer cells. Mutations in CD107b cause Danon disease, a lysosomal glycogen storage disorder.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis
• Surface staining in flow-based degranulation assays

Applications Tested:
Pre-titrated and tested for intracellular staining and flow cytometric analysis of Jurkat cells. Recommended usage: 5 µL (0.25 µg) per test (final volume 100 µL; 10⁵–10⁸ cells/test).

Optical Properties:

• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser

Filtration:
0.2 µm post-manufacturing filtered

Target Information

LAMP2 (CD107b) is a lysosome-associated membrane glycoprotein involved in lysosome biogenesis, fusion with phagosomes, and chaperone-mediated autophagy. It is a major constituent of lysosomal membranes, functioning in protection, maintenance, and adhesion. The mature form (~100–130 kDa) is extensively glycosylated. LAMP2 deficiency causes accumulation of autophagosomes, leading to cardiomyopathy and myopathy (Danon’s disease).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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