Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioABL-93 (ABL-93)), FITC, eBioscience
마우스 CD107b(LAMP-2)를 인식하는 단클론 항체로, FITC로 표지되어 세포 내 염색 및 유세포분석에 적합합니다. 0.5 µg/test 농도로 사용 가능하며, 488 nm 레이저에 최적화되어 있습니다. 리소좀 막 단백질 연구 및 탈과립 분석에 활용됩니다.
- 카탈로그번호
- 11-1072-81
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD107b (LAMP-2) Monoclonal Antibody (eBioABL-93 (ABL-93)), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 4 publications |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioABL-93 (ABL-93) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657579 |
Additional Formats
Product Specific Information
The eBioABL-93 monoclonal antibody reacts with mouse CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated protein (~120 kDa) expressed intracellularly in lysosomal membranes. It is transiently expressed on the surface of cytolytic T cells during degranulation, though less than CD107a, and may be identical to the mouse Mac-3 antigen.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
- Surface staining in flow cytometric degranulation assays
Applications Tested:
This antibody has been tested by intracellular staining and flow cytometric analysis of thioglycolate-elicited peritoneal exudate cells. It can be used at ≤0.5 µg per test (defined as the amount that stains a cell sample in 100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. It provides selectins with carbohydrate ligands and may play roles in tumor metastasis, lysosomal protection, and membrane adhesion. The unmodified LAMP2 polypeptide is approximately 46 kDa, while mature glycosylated forms range from 100–130 kDa. Structurally, LAMP2 consists of:
- A large amino-terminal intra-lysosomal domain
- A hydrophobic transmembrane domain
- A short cytoplasmic tail
LAMP2 participates in chaperone-mediated autophagy and lysosome-phagosome fusion. It exists in three variants (LAMP-2A, -2B, -2C). LAMP2 deficiency leads to autophagosome accumulation and is associated with Danon’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.