Thermo Fisher Scientific 6x His Tag Polyclonal Antibody, HRP
6x His 태그 단백질 검출용 HRP 결합 폴리클로날 항체. Western blot에 최적화되어 높은 특이성과 민감도를 제공. 항원 친화 크로마토그래피로 정제되어 신뢰성 높은 결과 보장. 연구용으로만 사용 가능.
- 카탈로그번호
- PA5141015
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific 6x His Tag Polyclonal Antibody, HRP
Applications
- Western Blot (WB): 1:2,000–1:5,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide immune sequence: HHHHHHHHC |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol, 0.2% BSA |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2932467 |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). The His tag is commonly expressed at either the N- or C-terminal region of recombinant proteins, allowing isolation or purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. This enables detection of proteins for which no specific antibody is available.
His-tagged proteins containing a stretch of histidine residues at the carboxyl or amino terminus are purified by metal chelate chromatography due to the metal-binding properties of histidine clusters. The affinity of the histidine-tag motif to Ni²⁺ ions allows selective purification to homogeneity using Ni²⁺-NTA adsorbents.
His tag-specific antibodies are used for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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