Thermo Fisher Scientific 6x His Tag Polyclonal Antibody
6x His 태그 단백질 검출용 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot 및 Immunoprecipitation에 적합. 고순도 항원 친화 크로마토그래피 정제 제품으로 안정적 보관 가능. 연구용으로만 사용.
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Thermo Fisher Scientific 6x His Tag Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide immune sequence: HHHHHHHHC |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932468 |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins for which no specific antibody is available. His-tagged proteins can be purified via metal chelate chromatography due to the metal-binding properties of histidine clusters.
For example, the strong and selective affinity of the His-tag motif to Ni²⁺ enables purification to homogeneity using Ni²⁺-NTA adsorbent.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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