Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700, Plus 594
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and is widely used as a marker for determining cell proliferation rates.

This antibody also recognizes human, non-human primate, and canine Ki-67.
Reported application: intracellular staining followed by flow cytometric analysis.

Tested Application: Intracellular staining of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Recommended use: ≤1.0 µg/test (100 µL final volume). Optimize antibody titration per assay.

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is suitable for cytometers with a violet (405 nm) laser.
Use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific polymer interactions when using multiple polymer dye-conjugated antibodies.
Not compatible with methanol-based fixation.

Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin regions and is absent in G0 cells. Because its expression correlates with cell proliferation, Ki-67 is widely used as a marker to assess tumor growth and cell cycling activity. The Ki-67 labeling index provides prognostic value in various cancer studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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