Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Violet 480, eBioscience
Armenian hamster IgG 단일클론 동형 대조 항체로, Brilliant Violet 480 형광체가 결합된 Flow Cytometry용 아이소타입 컨트롤입니다. 비특이적 결합 확인 및 배경 신호 구분에 적합하며, 405 nm 레이저 기반 장비에서 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBio299Arm |
| Conjugate | Brilliant Violet™ 480 |
| Excitation/Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925644 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605, 650, 711, 786
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.
Reported for use in flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes.
Use at the same concentration as the experimental antibody.
Brilliant Violet™ 480 (BV480) emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility before use.
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
Compatibility note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm Emission: 479 nm Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
Isotype control antibodies help distinguish non-specific background signal from specific antibody signal.
They have no relevant specificity to a target antigen and are used as negative controls in applications such as:
- Flow Cytometry
- Chromatin Immunoprecipitation (ChIP)
- Immunohistochemistry
- Gel Shift Assays
Isotype controls should match the species and isotype of the primary antibody for accurate comparison.
For directly labeled antibodies, use a control conjugated with the same label as the test antibody.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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