Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Alexa Fluor 488, 532, 561, 647
• APC, APC-eFluor 780
• Biotin, Brilliant UV 395, 496, 563, 615
• Brilliant Violet 421, 605, 711
• eFluor 450, 506
• FITC, Functional Grade
• Pacific Orange, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

This 2F1 monoclonal antibody reacts with mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer composed of glycosylated 30–38 kDa subunits and contains a cytoplasmic ITIM-like motif. It is expressed on approximately one-third of mouse NK cells and a subset of T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume)
• Recommended: Titrate carefully for optimal performance

Brilliant Violet™ 480 (BV480) emits at 479 nm and is suitable for cytometers with a violet (405 nm) laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (SB-4401-42)
• Brilliant Stain Buffer™ (00-4409-75)

Note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm | Emission: 479 nm | Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric type 2 transmembrane receptor with ITIM motifs that interact with SH2 domains of protein phosphatases such as SHP-1. It functions as an inhibitory receptor expressed on NK cells and certain T cells, which play roles in tumor and virus-infected cell lysis and immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지