Thermo Fisher Scientific CRYAB Monoclonal Antibody (1A7.D5), PE
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CRYAB 단백질을 인식하는 PE 결합 마우스 단클론 항체로, WB, ICC/IF, ELISA에 사용 가능. 보존제가 없는 액상 형태이며 4°C에서 보관. 높은 특이성과 감도로 알파 B 크리스탈린 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Pig, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A7.D5 |
| Immunogen | Native Alpha B Crystallin |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931655 |
Available Formats
- Unconjugated (MA5-27708)
- APC (MA5-45198)
- FITC (MA5-45199)
- PerCP (MA5-45200)
- Custom conjugation available upon request
Product Specific Information
- 0.5 µg/mL of MA5-45201 detects 50 ng purified alpha B crystallin by colorimetric immunoblot using Goat anti-mouse IgG:HRP as secondary antibody.
- Detects approximately 20 kDa (predicted molecular weight ≈ 21 kDa).
- No cross-reactivity with alpha A-crystallin, beta L-crystallin, beta H-crystallin, gamma-crystallin, HSP25, HSP27, or HSP47 proteins.
Target Information
Lens proteins are composed primarily of crystallins (≈95%), which maintain the proper refractive index of the lens. The mammalian lens includes three major classes: alpha, beta, and gamma crystallins.
- Alpha-crystallin: largest, composed of alpha-A and alpha-B subunits; accounts for 40% of total lens protein.
- Functions as a molecular chaperone, preventing aggregation and potential cataract formation.
- Phosphorylation may regulate structural and stress response functions.
- Alpha-B crystallin accumulation is associated with Alexander’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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