Thermo Fisher Scientific CRYAB Monoclonal Antibody (1A7.D5), FITC
CRYAB 단백질을 인식하는 FITC 결합 단클론 항체로, Western blot, ICC/IF, ELISA에 사용 가능. Bovine, Human, Pig, Rat 반응성. 단백질 G로 정제된 액상 형태이며, 4°C 암소 보관. 형광 분석용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Pig, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A7.D5 |
| Immunogen | Native Alpha B Crystallin |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.09 mM sodium bicarbonate/PBS, pH 7.2, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931653 |
Available Formats
- Unconjugated (MA5-27708)
- APC (MA5-45198)
- PE (MA5-45201)
- PerCP (MA5-45200)
- Custom conjugation available upon request
Product Specific Information
0.5 µg/mL of MA5-45199 was sufficient for detection of 50 ng purified alpha B crystallin by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 20 kDa (predicted molecular weight ~21 kDa).
Does not cross-react with alpha A-crystallin, beta L-crystallin, beta H-crystallin, gamma-crystallin, HSP25, HSP27, or HSP47 proteins.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%), which are also found in vertebrate skeletal muscle tissue.
Crystallins maintain the proper refractive index of the lens and include three major classes: alpha, beta, and gamma.
Alpha-crystallin, the largest, is composed of alpha-A and alpha-B gene products and makes up about 40% of total lens protein.
It also functions as a molecular chaperone, preventing aggregate formation that can lead to cataracts.
Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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