Thermo Fisher Scientific CD73 Polyclonal Antibody
Human 및 Mouse 시료에서 CD73 단백질 검출용 Rabbit Polyclonal Antibody. Western blot, IHC, ICC 등 다양한 응용 가능. 고순도 액상 형태로 제공되며 2 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 520–550 amino acids from the C-terminal region of human CD73 (NT5E) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion - Dialysis, Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2282968 |
Target Information
CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.
CD73 consists of a dimer of two identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage.
It is used as a marker of lymphocyte differentiation, and deficiency of CD73 occurs in various immunodeficiency diseases.
Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.
The CD73 gene has been localized to 6q14–q21 by immunofluorescence and hybrid studies.
Defects in the CD73 gene can lead to calcification of joints and arteries, and intestinal tuberculosis.
Two transcript variants encoding different isoforms of CD73 have been found.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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