Thermo Fisher Scientific MYOD Recombinant Rabbit Monoclonal Antibody (JM10-72)
Recombinant rabbit monoclonal antibody targeting Human MyoD1 (aa 36-80). Validated for WB, Flow Cytometry, and IP. Offers high specificity, lot-to-lot consistency, and animal origin-free formulation. Suitable for muscle differentiation research.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM10-72 |
| Immunogen | Synthetic peptide within Human MyoD1 aa 36–80 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809918 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
The Myogenic determination gene (MyoD) was first identified by its ability to convert embryonic mouse fibroblast cells to muscle cells. Forced expression of MyoD (human homolog myf3) can induce muscle cell formation or activate transcriptionally inactive muscle-specific genes.
The regulatory domain of MyoD lies within a 70 amino acid region containing a basic DNA-binding motif and a helix-loop-helix (HLH) dimerization motif.
Other related genes with homology in the HLH domain include:
- myf5
- myogenin (human homolog myf4)
- myf6 (also known as MRF4 and herculin)
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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