Thermo Fisher Scientific VEGF Monoclonal Antibody (16F1)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

The VEGF-A monoclonal antibody clone #16F1 (Product #M808) has been successfully paired as the coating antibody in a sandwich ELISA with detection antibody P802B (biotinylated conjugate of Product #P802).
Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125–0.5 µg/mL for detection.

Target Information

VEGF (vascular endothelial growth factor) is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis, promoting tumor progression and metastasis. VEGF enhances endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability.

The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system are key regulators in angiogenesis signaling. Most tumors require angiogenesis, with VEGF being the most potent angiogenic cytokine.

Five isoforms of VEGF arise from alternative splicing of a single gene, binding to tyrosine kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), primarily expressed on endothelial cells. These receptors are critical for embryonic vascular development.

An alternatively spliced form, sFLT1, binds VEGF with high affinity and inhibits angiogenesis. Elevated VEGF levels are associated with POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes), also known as Crow-Fukase syndrome, affecting multiple organs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.