Thermo Fisher Scientific EGF Monoclonal Antibody (3A8), Biotin

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Request custom conjugation

Product Specific Information

The M806B anti-EGF antibody (biotinylated conjugate of clone 3A8) has successfully been paired as the detection antibody in a sandwich ELISA with coating antibody M805 (Clone 1H11).
Typical dilutions for sandwich ELISA:

• Coat: 1–3 µg/mL
• Detection: 0.125–0.5 µg/mL

Target Information

Epidermal Growth Factor (EGF) binds to the EGF receptor (EGFR), a 170 kDa protein kinase, activating diverse cellular pathways. Upon ligand binding, EGFR forms homo- or heterodimers, initiating distinct signaling cascades through SH2-containing effector proteins.
EGF is widely expressed in kidney, brain, prostate, and salivary glands, acting as a potent mitogenic factor. The phosphorylated receptor recruits adapter proteins such as GRB2, activating downstream signaling modules including:

• RAS–RAF–MEK–ERK
• PI3K–AKT
• PLCγ–PKC
• STAT pathways

Research suggests EGF also influences NF-κB signaling. Mutations in the EGF gene are linked to hypomagnesemia type 4, and dysregulation is associated with cancer progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.