Thermo Fisher Scientific Fen1 Polyclonal Antibody
Rabbit 유래의 Polyclonal 항체로, 인간 FEN1 단백질의 250-300번 잔기 영역을 면역원으로 제작됨. Western blot, IHC, IP 등 다양한 응용에 적합. 액상 형태로 1 mg/mL 농도이며, 4°C 보관 및 Wet ice 배송. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Item | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 250 and 300 of human Flap Structure Endonuclease 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections. Incubate for 16–18 hours.
- Based on 100% sequence identity, this antibody is predicted to react with Sheep and Bovine.
Target Information
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein functioning as an endonuclease that cleaves branched DNA structures containing single-stranded 5′ flaps or as an exonuclease on DNA nicks and gaps. Its activities are stimulated by association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 plays essential roles in DNA replication and repair and is required in cell-free DNA replication systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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