Thermo Fisher Scientific EpCAM Monoclonal Antibody (OTI2C8), TrueMAB
EpCAM 단백질을 표적하는 Mouse IgM 단클론 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 인간 시료에 반응하며, 고순도의 친화 크로마토그래피 정제 제품입니다. PBS/BSA/glycerol buffer에 보관되며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 24–265 of human EpCAM produced in E. coli. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ep-CAM (Epithelial Cell Adhesion Molecule, also known as ESA or CD326) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent homotypic cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM correlates with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for tumors of epithelial origin. It is localized mainly on the basolateral surface of simple epithelia and various carcinoma types, and can help distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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