Thermo Fisher Scientific RIM3 Polyclonal Antibody
RIM3 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC에 사용 가능. Human과 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS buffer에 0.02% sodium azide 포함, 2–8°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 17 amino acid peptide from near the amino terminus of human Rim3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11156597 |
Product Specific Information
- A suggested positive control is human brain tissue lysate.
- PA5-20475 can be used with blocking peptide PEP-0595.
Target Information
Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neural transmission and plasticity.
While both Rim1 and Rim2 are thought to be effector proteins for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner, less is known of Rim3.
Expression of Rim3 in PC12 cells induced a significant increase in calcium-triggered exocytosis, with no appreciable change in the baseline release, suggesting that it plays a role in the regulation of exocytosis.
Rim3 protein localizes primarily to neuronal dendrites and the postsynaptic densities, as opposed to Rim1 which is found in presynapse locations, indicating that Rim3 may contribute to synapse transmission and plasticity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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