Thermo Fisher Scientific BTLA Monoclonal Antibody (OTI2E4), TrueMAB
BTLA 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western Blot, IHC(P), Flow Cytometry에 사용 가능. 인간 시료 반응성. 동결건조 형태로 제공되며, 친화 크로마토그래피로 정제. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E4 |
| Immunogen | Full-length human recombinant protein of human BTLA produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
BTLA (B and T-lymphocyte attenuator) is a co-inhibitory receptor belonging to the CD28 superfamily. It acts as a lymphocyte inhibitory receptor regulating immune responses. BTLA is constitutively expressed on most CD4+ and CD8+ T cells, with expression decreasing upon T cell activation. It remains expressed on Th1 cells but not Th2 cells.
BTLA interacts with the TNF receptor superfamily member HVEM, forming an important pathway for lymphocyte activation and immune homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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