Thermo Fisher Scientific MAP2K2 Monoclonal Antibody (OTI1A2), TrueMAB
MAP2K2 단백질을 인식하는 Thermo Fisher Scientific의 단일클론 항체로, WB, IHC, ICC 등 다양한 응용에 사용 가능. Mouse IgG2a 형식이며, HEK293T 세포에서 생산된 MAP2K2 전체 길이 항원을 면역원으로 사용. 고순도 친화 크로마토그래피 정제, 동결건조 형태, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A2 |
| Immunogen | Full length human recombinant protein of human MAP2K2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction. It activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop. MEK1/2 are activated by dual phosphorylation on serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, providing a negative feedback mechanism that suppresses MEK1 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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