Thermo Fisher Scientific TRPC6 (extracellular) Polyclonal Antibody
TRPC6 단백질의 세포외 영역을 인식하는 Rabbit Polyclonal 항체. Western blot, ICC/IF, Flow cytometry에 사용 가능. 항원 친화 크로마토그래피로 정제. PBS 기반 완충액에 1% BSA, 0.05% sodium azide 포함. -20°C 보관, 반복 동결·해동 피해야 함.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 2.5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)DNVKYYNLARIKWD, corresponding to amino acid residues 573–586 of mouse TRPC6 (2nd extracellular loop) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.85 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution:
- Add 25 µL, 50 µL, or 0.2 mL double-distilled water (DDW), depending on sample size.
- Ships as lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- Reconstituted solution can be stored at 4°C for up to 1 week.
- For long-term storage, aliquot and store at -20°C.
- Avoid multiple freeze-thaw cycles.
- Centrifuge antibody preparations before use (10,000 × g, 5 min).
Target Information
TRPC6 forms a receptor-activated calcium channel in the cell membrane.
It is activated by diacylglycerol (DAG) and regulated through a phosphatidylinositol second messenger system, independent of protein kinase C and intracellular calcium levels.
Mutations in TRPC6 are associated with focal segmental glomerulosclerosis 2 (FSGS2).
TRPC6 belongs to the “canonical TRP” (TRPC) family, part of the mammalian transient receptor potential (TRP) superfamily.
Members of the TRPC3/6/7 subfamily are activated by DAG analogs, suggesting regulation via PLC-coupled receptors.
TRPC6 functions as a non-selective, store-independent, receptor-activated cation channel and is insensitive to IP3 activation.
It encodes endogenous DAG-activated receptor-operated cation channels in vivo.
Diseases associated with TRPC6 include:
- Glomerulosclerosis, Focal Segmental, 2
- Focal Segmental Glomerulosclerosis
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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