Thermo Fisher Scientific CD91 Monoclonal Antibody (A2MR-a2), NovaFluor Yellow 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.1 µg)/test

Product Specifications

Product Specific Information

The A2MR-a2 monoclonal antibody reacts with human CD91, also known as LRP-1 and alpha-2 macroglobulin receptor. CD91 is a 600 kDa type I transmembrane protein consisting of alpha and beta chains. The alpha chain binds multiple ligands, while the beta chain contains tyrosine phosphorylation motifs that interact with adaptor and signaling proteins. CD91 mediates endocytosis, lysosomal trafficking, and antigen cross-presentation via MHC class I molecules. It is expressed in monocytes, dendritic cells, hepatocytes, fibroblasts, adipocytes, neurons, and syncytiotrophoblasts.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of normal human peripheral blood cells. Recommended usage: 5 µL (0.1 µg) per test in 100 µL final volume. Cell number range: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls: add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Conjugates:
Based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimized fluorescence signatures with minimal spillover and spread.

Excitation: 552 nm
Emission: 663 nm
Laser: 561 nm (Yellow)

Target Information

The encoded protein functions as an endocytic receptor involved in intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. It is essential for A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques in Alzheimer's disease. Expression decreases with age and is reduced in Alzheimer’s brain tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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