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Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Ultra Violet 395, eBioscience
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Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Ultra Violet 395, eBioscience

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마우스 Ly-6G(Gr-1) 항원을 인식하는 단클론 항체로, 중성구 표지에 특이적입니다. Brilliant Ultra Violet 395 형광체 결합으로 UV 레이저 기반 유세포분석에 적합합니다. 1.0 µg/test 농도로 사용 가능하며, 비특이적 결합 최소화를 위해 Stain Buffer 사용을 권장합니다.

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Thermo Fisher Scientific 363-9668-82 Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Ultra Violet 395, eBioscience 100 ug pk판매 단위 pk
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868,100원VAT 포함 954,910원
Thermo Fisher Scientific 363-9668-80 Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Ultra Violet 395, eBioscience 25 ug pk판매 단위 pk
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316,800원VAT 포함 348,480원

Thermo Fisher Scientific · Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

Application Tested Dilution
Flow Cytometry (Flow) 1.0 µg/test

Product Specifications

항목 내용
Species Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Recommended Isotype Control Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Ultra Violet™ 395, eBioscience™
Class Monoclonal
Type Antibody
Clone 1A8-Ly6g
Conjugate Brilliant Ultra Violet™ 395
Excitation/Emission Max 347 / 399 nm
Form Liquid
Concentration 0.2 mg/mL
Purification Affinity chromatography
Storage Buffer PBS, pH 7.2, with BSA
Contains 0.09% sodium azide
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Ambient (domestic); Wet ice (international)
RRID AB_2925308

Additional Available Formats

  • Alexa Fluor 700
  • APC
  • APC-eFluor 780
  • Biotin
  • Brilliant UV 563, 661, 737
  • Brilliant Violet 421, 480, 650, 786
  • eFluor 450
  • FITC
  • Functional Grade
  • PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
  • PerCP-eFluor 710
  • Super Bright 436, 600, 702
  • Request custom conjugation

Product Specific Information

Description:
Monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. It plays a role in neutrophil infiltration, recruitment, and migration, and associates with β2 integrins (CD11a, CD11b). The ligand for Ly-6G is currently unknown.

Unlike RB6-8C5, the 1A8-Ly6g antibody binds specifically to Ly-6G on neutrophils and has been used for neutrophil depletion with reported antagonist activity.

Applications Reported:

  • Flow cytometric analysis of mouse bone marrow cells.

Applications Tested:

  • Use ≤ 1.0 µg/test (100 µL final volume).
  • Optimal cell number: 10⁵–10⁸ cells/test.
  • Titrate for optimal performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is designed for cytometers with a 355 nm UV laser. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

Excitation / Emission / Laser:
350 nm / 395 nm / Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.


Target Information

Ly6G (Lymphocyte antigen 6 complex locus G6D):
A component of the myeloid differentiation antigen Gr-1, along with Ly6C.

  • Molecular weight: ~21–25 kDa
  • Expressed by myeloid-derived cells in bone marrow
  • Marker for peripheral neutrophils, monocytes, and granulocytes
  • Expression correlates with granulocyte differentiation and maturation
  • Involved in anti-tumor response and may inhibit local immune responses

For Research Use Only.
Not for diagnostic use or resale without authorization.


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