Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Ultra Violet 395, eBioscience
마우스 Ly-6G(Gr-1) 항원을 인식하는 단클론 항체로, 중성구 표지에 특이적입니다. Brilliant Ultra Violet 395 형광체 결합으로 UV 레이저 기반 유세포분석에 적합합니다. 1.0 µg/test 농도로 사용 가능하며, 비특이적 결합 최소화를 위해 Stain Buffer 사용을 권장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 1.0 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Ultra Violet™ 395, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | Brilliant Ultra Violet™ 395 |
| Excitation/Emission Max | 347 / 399 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925308 |
Additional Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 563, 661, 737
- Brilliant Violet 421, 480, 650, 786
- eFluor 450
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 702
- Request custom conjugation
Product Specific Information
Description:
Monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. It plays a role in neutrophil infiltration, recruitment, and migration, and associates with β2 integrins (CD11a, CD11b). The ligand for Ly-6G is currently unknown.
Unlike RB6-8C5, the 1A8-Ly6g antibody binds specifically to Ly-6G on neutrophils and has been used for neutrophil depletion with reported antagonist activity.
Applications Reported:
- Flow cytometric analysis of mouse bone marrow cells.
Applications Tested:
- Use ≤ 1.0 µg/test (100 µL final volume).
- Optimal cell number: 10⁵–10⁸ cells/test.
- Titrate for optimal performance.
Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is designed for cytometers with a 355 nm UV laser. Ensure instrument compatibility.
Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:
- Super Bright Complete Staining Buffer (SB-4401-42)
- or Brilliant Stain Buffer™ (00-4409-75)
Excitation / Emission / Laser:
350 nm / 395 nm / Ultraviolet Laser
Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D):
A component of the myeloid differentiation antigen Gr-1, along with Ly6C.
- Molecular weight: ~21–25 kDa
- Expressed by myeloid-derived cells in bone marrow
- Marker for peripheral neutrophils, monocytes, and granulocytes
- Expression correlates with granulocyte differentiation and maturation
- Involved in anti-tumor response and may inhibit local immune responses
For Research Use Only.
Not for diagnostic use or resale without authorization.
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