Thermo Fisher Scientific Phospho-CDK9 (Thr186) Polyclonal Antibody
Phospho-CDK9 (Thr186) 폴리클로날 항체로 인간, 마우스, 랫트에 반응하며 다양한 응용(서던블롯, IHC, ICC, Flow, ELISA)에 사용 가능. KLH 결합 합성 인산화 펩타이드 기반 면역원. 고순도 Protein A 정제, 액상 형태, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human CDK9 around the phosphorylation site of Thr186 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CDK9 is a serine/threonine kinase which, when complexed with CyclinT1, forms an active holoenzyme complex that regulates the eukaryotic cell cycle. CDK9 regulates cytokine-inducible transcription networks by facilitating promoter recognition of target transcription factors. It promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also exhibits kinase activity toward the CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and predisposition to heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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