Thermo Fisher Scientific Phospho-cdc25C (Ser216) Polyclonal Antibody
Phospho-cdc25C (Ser216) 특이적 폴리클로날 항체로, 인간·마우스·랫에서 반응. WB, IHC, ICC, ELISA 등 다양한 응용 가능. Protein A로 정제된 액상형 항체이며, -20°C 보관. 세포 분열 조절 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
M-phase inducer phosphatase 3 is an enzyme encoded by the CDC25C gene in humans. This gene is highly conserved and plays a key role in cell division regulation. The encoded protein is a tyrosine phosphatase belonging to the Cdc25 phosphatase family, which dephosphorylates cyclin B-bound CDC2 (CDK1) to trigger mitosis. It may also suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants have been described, though many are not fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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