Thermo Fisher Scientific AIF Polyclonal Antibody
Apoptosis-Inducing Factor(AIF)를 표적하는 Rabbit Polyclonal Antibody로 다양한 응용(WB, IHC, IP)에 적합. 인간 AIF(잔기 100–150) 영역을 면역원으로 사용. 액상 형태, 0.2 mg/mL 농도. 연구용으로만 사용 가능.
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Thermo Fisher Scientific AIF Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and 150 of human Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation triggered by activation of caspase family members and other proteins.
AIF localizes in mitochondria and translocates to the nucleus upon induction of apoptosis, promoting release of apoptogenic proteins such as cytochrome c and caspase-9. It induces chromatin condensation and large-scale DNA fragmentation, hallmarks of apoptosis, both in isolated nuclei and in live cells.
AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, resulting in severe mitochondrial encephalomyopathy. Alternative splicing generates multiple transcript variants; a related pseudogene exists on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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