Thermo Fisher Scientific AIF Polyclonal Antibody
Apoptosis-Inducing Factor(AIF)에 특이적인 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, IP 등 다양한 응용에 적합하며, Mouse와 Rat에도 반응 예측됨. 액상 형태로 4°C 보관, 1년의 권장 유효기간.
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Thermo Fisher Scientific AIF Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 563 and 613 of human Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
Apoptosis-Inducing Factor (AIF) is involved in chromatin condensation and DNA fragmentation during apoptosis. AIF localizes in mitochondria and translocates to the nucleus upon induction of apoptosis, promoting the release of apoptogenic proteins such as cytochrome c and caspase-9. It is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, leading to severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants, and a related pseudogene is identified on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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